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Prabhakar, C. S.
- Field Evaluation of an Indigenous Granulosis Virus Isolate for Pieris brassicae (Linnaeus) Management under North Western Himalayan Conditions
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Authors
Affiliations
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 176062, Himachal Pradesh, IN
2 KVK Mandi, Sundernagar, 175019, Himachal Pradesh, IN
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 176062, Himachal Pradesh, IN
2 KVK Mandi, Sundernagar, 175019, Himachal Pradesh, IN
Source
Journal of Biological Control, Vol 25, No 3 (2011), Pagination: 217-222Abstract
A granulosis virus strain infecting Pieris brassicae (PbGV) was isolated from the dry temperate region of Northwestern Himalayas as a potential microbial agent for the management of P. brassicae. Host specificity and safety to parasitoid Cotesia glomerata (L.) revealed high host specificity and safety to the most prevalent natural enemy. Field evaluation of PbGV was carried out alone and in combination with another microbial pesticide formulation pathogenic to lepidopteran pests viz. Bacillus thuringiensis (DIPEL®) and neem seed kernel extract (NSKE) on cole crops (cabbage, cauliflower and broccoli) at two geographically isolated locations viz. Palampur (sub tropical) and Sangla (dry temperate) in Himachal Pradesh, India in order to design ecofriendly management modules in the hill state, which is fast transforming in to organic farming. The studies revealed that the PbGV isolate alone was quite effective against P. brassicae larvae at higher dose of 1.12 × 1012 OBs/ha per hectare whereas at lower dose of 5.58 × 10" LE/ha, PbGV was effective when combined with Bt or NSKE. Single foliar application of PbGV was sufficient to suppress the pest at higher altitude areas (dry temperate region) while two applications at 15 days interval were needed at low altitude areas. The findings are of great significance for exploitation of its full potentialKeywords
PbGV, Pieris brassicae, Granulosis Virus, Cabbage Butterfly.Full Text
References
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- Sood, P., Mehta, P. K., Bhandari, K. and Prabhakar, C. S. 2010. Transmission and effect of sublethal infection of granulosis virus (PbGV) on Pieris brassicae Linn. (Pieridae: Lepidoptera). Journal of Applied Entomology, 134: 774– 780.
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- Molecular Characterization of Pieris brassicae Granulosis Virus (pbGV) from the Himalayan Region of India
Abstract Views :241 |
PDF Views:125
Authors
Affiliations
1 Crop Research Sub Station, CSK Himachal Pradesh Krishi Vishvavidyalaya, Sundernagar, Mandi, Himachal Pradesh - 175 019, IN
2 ICAR Research Complex for Eastern Region Research Centre, Plandu, Ranchi - 834 010, Jharkhand, IN
1 Crop Research Sub Station, CSK Himachal Pradesh Krishi Vishvavidyalaya, Sundernagar, Mandi, Himachal Pradesh - 175 019, IN
2 ICAR Research Complex for Eastern Region Research Centre, Plandu, Ranchi - 834 010, Jharkhand, IN
Source
Journal of Biological Control, Vol 26, No 2 (2012), Pagination: 37-43Abstract
A strain of granulosis virus from Pieris brassicae was isolated from the dry temperate region of Himachal Pradesh, India situated at an altitude of 2580 m above msl. The molecular characterization of this strain of PbGV was carried out with granulin gene nucleotide sequence analysis. The nucleotide sequence of 404 bp of PbGV was submitted to GenBank, NCBI with accession number FJ151541. Nucleotide and phylogenetic analysis confirm this isolate as Pieridae (insect family) infecting granulovirus with lowest genetic distance of 0.012, 0.015, 0.016 with other Pieris rapae and Pieris brassicae granulosis viruses. More number of isolates and other molecular markers, however, would be useful to understand the phylogenetic relationship of this Indian isolate of PbGV.Keywords
Granulosis Virus, Pieris brassicae, Granulin and Phylogeny.Full Text
References
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- Molecular Diversity and Antibiotic Sensitivity of Gut Bacterial Symbionts of Fruit Fly, Bactrocera tau Walker
Abstract Views :242 |
PDF Views:145
Authors
Affiliations
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh, IN
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh, IN
Source
Journal of Biological Control, Vol 23, No 3 (2009), Pagination: 213-220Abstract
Random amplified polymorphic DNA-Repetitive extragenic palindromic (RAPD- REP) PCR-restriction length polymorphism (PCR-RFLP) were used to study the genetic similarity among bacterial symbionts of fruit fly, Bactrocera tau Walker. RAPD-PCR and REP-PCR placed three bacteria into two groups, viz., group A (BC1 and BC3) and group B (BC2). However, PCR- RFLP analysis of recA and rrs genes of bacterial symbiont placed BC1 and BC2 into group A and BC3 into group B. Studies on antibiotic sensitivity pattern also supported the results of PCR-RFLP as the response of BC1 and BC2 was similar to different antibiotics. All the bacterial symboints were found to be sensitive to co-trimoxazole, gentamycene and nofloxacin. The findings suggest that there may be a possibility of utilising these antibiotics for disruption of fruit fly biology as foliar symbioticides for their management.Keywords
Bacterial Symbionts, Bactrocera tau, recA gene, rrs gene, RAPD and REP–PCR Analysis.Full Text
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